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What is a Western Blot and how does it work?

Understanding the Principles of Western Blots: Learn the fundamental principles of Western blotting, including its purpose, procedure, and components, and how it is used to detect specific proteins. Steps Involved in Western Blots: Familiarize yourself with the step-by-step process of Western blotting, from sample preparation to protein detection, and understand the importance of each step in obtaining reliable results. Quantification of Protein Amounts and Sizes: Learn how to quantify relative amounts based on band intensity measurement and how the protein sizes can be estimated using a molecular weight marker. Example Involving Protein Cleavage: Explore an example of Western blotting involving protein cleavage, such as proteolytic processing, to understand how this technique can be used to study post-translational modifications and protein processing events.