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DIC Microscopy | Differential interference contrast | DIC vs Phase contrast.

DIC is also termed as Differential interference contrast Microscope. This microscopy technique uses the polarizer and a special type of prism to split light into two rays (or two waves) known as the e ray and the o ray. Both the rays are separated by a small distance known as the shear distance. There is also a phase difference between both the rays. Both the rays passes through the specimen and further hits the second prism which combines them into a single ray. finally the image observed has one bright region and other dark region. the physics involved in DIC microscope has been discussed in detail. Finally we also compare DIC and phase contrast microscope as both of them can be confusing.