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#drnajeeb #medicaleducation #drnajeeblectures #spleen Elisa Test | Direct Indirect & Sandwich 🩸 Like this video? Sign up now on our website at https://www.DrNajeebLectures.com to access 800+ Exclusive videos on Basic Medical Sciences & Clinical Medicine. These are premium videos (NOT FROM YOUTUBE). All these videos come with English subtitles & download options. Sign up now! Get Lifetime Access for a one-time payment of $99 ONLY! Sign up now on our website at https://members.drnajeeblectures.com/... --------------------------------------------------------------------------------------------------------------------------- Why sign up for premium membership? Here's why! Membership Features for premium website members. 1. More than 800+ Medical Lectures. 2. Basic Medical Sciences & Clinical Medicine. 3. Mobile-friendly interface with android and iOS apps. 4. English subtitles and new videos every week. 5. Download option for offline video playback. 6. Fanatic customer support and that's 24/7. 7. Fast video playback option to learn faster. 8. Trusted by over 2M+ students in 190 countries. --------------------------------------------------------------------------------------------------------------------------- ▬▬▬▬▬▬▬▬▬▬ Contents of this video ▬▬▬▬▬▬▬▬▬▬ 00:00:00 What Is ELISA? 00:00:40 Explanation Of Mechanism Through An Example 00:09:57 Addition Of Substrate 00:11:55 Advantages Of Direct ELISA Test 00:13:58 Issues And Modifications Of ELISA 00:19:50 What Is Epitope? 00:22:01 Indirect Elisa Test And Its Mechanism 00:26:50 Performance Of Direct And Indirect Elisa Test 00:33:48 Effect Of Adding More Enzymes 00:34:40 When To Use Direct Or Indirect Test 00:39:44 Purpose Of Using These Two Tests Together 00:42:32 Sandwich Modification 00:49:37 Difference Between Direct And Direct Sandwich Elisa 00:51:03 Indirect Sandwich Test 00:54:27 Rapid Summary 00:58:07 Question 01:01:25 Radioimmuno Assay And Its Comparison With ELISA --------------------------------------------------------------------------------------------------------------------------- The ELISA test is simple and straightforward. You’ll probably need to sign a consent form, and your doctor should explain the reason for doing the test. The ELISA test involves taking a sample of your blood. First, a healthcare provider will cleanse your arm with an antiseptic. Then, a tourniquet, or band, will be applied around your arm to create pressure and cause your veins to swell with blood. Next, a needle will be placed in one of your veins to draw a small sample of blood. When enough blood has been collected, the needle will be removed and a small bandage will be placed on your arm where the needle was. You’ll be asked to maintain pressure at the site where the needle was inserted for a few minutes to reduce blood flow. This procedure should be relatively painless, but your arm may throb a little after it’s done. The blood sample will be sent to a laboratory for analysis. In the lab, a technician will add the sample to a petri dish containing the specific antigen related to the condition for which you are being tested. If your blood contains antibodies to the antigen, the two will bind together. The technician will check this by adding an enzyme to the petri dish and observing how your blood and the antigen react. You may have the condition if the contents of the dish change color. How much change the enzyme causes allows the technician to determine the presence and amount of antibody --------------------------------------------------------------------------------------------------------------------------- Join this channel to get access to perks: Sign up now on our website at https://members.drnajeeblectures.com/... / @doctornajeeb Follow us on Facebook :- / drnajeeb Follow us on Instagram :- / drnajeeblectures