Скачать с ютуб LabTips: prepping lots of gel samples edition в хорошем качестве

LabTips: prepping lots of gel samples edition

Random (for least you a) lab tip(s) for preparing lots of SDS-PAGE samples - instead of preparing them in individual tubes, use PCR strip tubes or PCR plates - instead of adding loading buffer to each sample individually, pipette excess into a PCR strip or plate row, then use a multichannel to load into the wells (either before adding sample, or after sample (then you can mix multi as well)) - if you’re running multiple gels in the same box, label the gel cassettes (at the top of the plates, I suggest writing the name multiple times in case it washes/rubs off) - if you’re running multiple gels, load all the ladders first and it will help you find the other wells (and is helpful because you often load a lower volume of ladder and then you don’t have to keep changing the pipet setting) - if you’re running 4 gels in a tetra box, make sure that you see bubbles from each module - sometimes the connection can be bad and you need to fiddle around to make sure all the metals are making contact to the power supply more on SDS-PAGE: http://bit.ly/sdspageruler more on working with lots of samples: http://bit.ly/mastermixesmultichannels more (hopefully) helpful random practical lab tips & tricks: https://bit.ly/lab_tricks_page      more about all sorts of things: #365DaysOfScience All (with topics listed) 👉 http://bit.ly/2OllAB0 or search blog: http://thebumblingbiochemist.com