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Recombinant Protein Expression in Ecoli

http://technologyinscience.blogspot.c... Recombinant Protein expression in E.coli, Best suitable strains for protein expression, advantages of using E.coli for choosing the host for protein expression. 0:00 Introduction 0:07 Protein Expression in E.coli 0:12 Advantages of E.coli System 1:45 E.Coli Strain & Application 2:23 Vectors for Protein Expression & Features 2:53 pUC19 Vector 3:08 Most widely used vectors 3:13 Cloning & Screening 3:26 Plasmid Extraction / Isolation 4:21 Restriction Digestion & Ligation 5:08 Restriction Digestion and Gel 5:15 Transformation and Expression 5:21 Transformation - Procedure 6:20 Shake Flask Culture- Protein Expression & Analysis 6:43 Lac Operon & IPTG Induction 7:43 Protein Analysis - SDS PAGE 8:01 Process Scale Up 8:08 Protein Characterization