Скачать с ютуб Extraction of Lactose and Casein from milk в хорошем качестве

Extraction of Lactose and Casein from milk

The procedure for extracting Casein and Lactose from milk is demonstrated here For this experiment I have used skimmed sweet milk. Materials required Skimmed sweet cows milk - 100ml 1N HCl (or any other acid) - 10ml Calcium carbonate - 4g Activated Charcoal - 2g Methanol/Ethanol - 100ml Milk is a complex colloid containing a lot of substances like Water,fat,casein,whey proteins,lactose and minerals. Principle : Casein precipitated by acid filtered off Excess acid neutralised by calcium carbonate Solution boiled to precipitate out whey proteins and then filtered. Boil solution to make it 1/4 then add charcol to decolourise Then add alcohol ( since lactose is more soluble in alcohol) Procedure Take 100ml of skimmed milk in a beaker. If you are using regular milk just remove the fat floating on top by heating the milk to boil . Now add 10N HCl. While adding the acid there should be constant stirring ,only then the acid will penetrate down the precipitated casein,otherwise the casein precipitated on top act as a barrier preventing the acid from reacting with the bottom layer. Now filter the solution to get the precipitated casein out of the solution. I just used gravity filtration method. Vaccum filtration will be much faster . Only casein has got precipitated here because it's the negatively charged molecule and it's charges get neutralised on adding the acid which made it to seperate out from the colloid . The clear solution obtained after filtration is called whey. It still contain some other proteins like lactalbumin and they are called as whey protein. Now we add the calcium carbonate to remove the acid .We did that because the acid present in it can decompose the lactose in the next step of heating it. To remove all the other soluble proteins we boil the solution so that the proteins get denatured and precipitate out from the colloid. Now ultimately filter the solution and then boil the solution to about 1/4th . The solution will now have some nasty dark color. We will add some activated charcoal and keep it for 30min so most of it will get decolourised. Now we add the alcohol and mix it up well and filter to remove the charcoal and any other insoluble residue. We now let the solution sit in direct sunlight so that alcohol will get vapourised and beautiful crystals lactose will get crystalised. Collect the crystals ,dry them and dissolve them once again in little amount of alcohol and allow the alcohol to vapourise and recrystallise lactose. Weigh lactose and the casein which we made in the initial part . So that's pretty much it. Hope you like my notes Please Subscribe to my channel This video has been recorded and edited with much effort and difficulty. Lack of proper equipment and chemicals can make video look unfinished. I request your love and support in donations also. Links below 👇 Support me on patreon   / vibzzlab   Or Support me via paypal https://www.paypal.me/vibzzlab Or Support on Google pay Vibin Chungath If you have any suggestions on new videos , modification to be done or queries regarding any content or anything you don't understand or complaints regarding any of the contents, You can email me Email id : [email protected] I wish to plan a community Report your views in comment section here or email me. Intro video courtesy Syn Cole - Feel Good [NCS Release] Connect with NCS: Snapchat: ncsmusic •   / nocopyrightsounds   •   / nocopyrightsounds_   •   / nocopyrightsounds   •   / ncsounds   • http://spoti.fi/NCS Syn Cole •   / syncole   •   / syncole   •   / syncoleofficial   •   / syncole