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How to Set up HPLC calibration curve - External Standard Calibration Method 1 год назад


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How to Set up HPLC calibration curve - External Standard Calibration Method

How to Set up HPLC Calibration Curve - External Standard Calibration Method is demonstrated in this video. A sample of unknown concentration containing a mixture of compounds is analysed using the Shimadzu i-Series LC-2050C 3D, which includes a photodiode array (PDA) detector. We will also demonstrate how to accurately prepare a set of standards for external calibration in order to obtain a good calibration curve. This video provides a step-by-step demonstration of how to operate the Shimadzu LabSolutions software (real time analysis and post run analysis) in order to obtain a calibration curve, starting from setting up a method file to run a real time analysis all the way to processing the data in the post-run LabSolutions analysis window. Shimadzu i-Series LC-2050C 3D: https://www.shimadzu.com/an/products/... Photodiode Array Detector: https://www.shimadzu.com/an/service-s... #HPLC #HPLC_calibration_curve #HPLC_analysis¬_for_unknown_sample

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