Скачать с ютуб Yeast cloning vectors - YIp, YEp, YRp, YCp, YACs - Shuttle plasmid vectors в хорошем качестве

Yeast cloning vectors - YIp, YEp, YRp, YCp, YACs - Shuttle plasmid vectors

References/Resources:   / the_crux   This video discusses the general biology of yeast cloning vectors. There are 5 classic vectors - Integrating, Episomal, Replicating, Centromeric, and Artificial Chromosomes - as well as the naturally occurring Yeast 2 mu plasmid. Yeast vectors rely on coli-based vectors like pBR322, pUC19, or Bluescripts. Typically, yeast cloning vectors tend to be suboptimal due to the complexity of the organism (vs. bacteria). This video highlights how these vectors can be used, their advantages, and disadvantages. Jump to your favorite section: 00:00 Cloning in Yeast 01:11 Shuttle vectors 01:54 Auxotrophy 03:33 Summary of Yeast vectors 04:53 YIps 08:28 YEps 10:43 YRps 13:48 YCps 15:25 YACs (Linear) 18:34 YACs are terrible! 20:02 YACs (Circular) Patreon Page:   / the_crux   Suggested videos: Plasmids:    • Plasmid and Phagemid cloning vector -...   Yeast (ARS):    • DNA Replication - Origin of Replicati...   BACs:    • F plasmid, BACs, Fosmid cloning vecto...   Must watch playlists All videos on Genetic Engineering:    • Genetic Engineering (Genes to Genomes)   All videos on DNA Replication:    • All about DNA Replication!   All videos on Transcription:    • All about Transcription!   All videos on Translation:    • All about Translation!   Consider supporting the growth of this channel by becoming a Patron:   / the_crux   I appreciate your support :)