Скачать с ютуб How to Speed up Your PBMC Isolations with SepMate™ в хорошем качестве

How to Speed up Your PBMC Isolations with SepMate™

This in-depth technical video demonstrates how to use SepMate™ tubes to isolate peripheral blood mononuclear cells (PBMCs) from whole blood or bone marrow in just 15 minutes. 0:00 Introduction 0:17 Protocol overview: PBMC isolation with standard density gradient with non- SepMate™ tubes vs isolation with SepMate™ tubes 0:32 SepMate™ tube features Protocol walkthrough 1:46 What is needed for PBMC isolation with SepMate™? 2:33 Addition of density gradient media to the SepMate™ tube 4:10 Blood sample dilution 5:49 Addition of the diluted blood sample to the SepMate™ tube 7:07 Centrifugation of SepMate™ tube 8:50 Collection of isolated PBMCs 11:39 First wash of isolated PBMCs 13:55 Second wash of isolated PBMCs 16:01 Comparison of washed PBMC pellet after isolation with standard density gradient protocol with a non- SepMate™ tube Learn more about SepMate™ PBMC isolation tubes at http://www.sepmate.com #PBMCisolation #CellSeparation #CellIsolation For a full list of products, as well as educational resources, visit our website: https://www.stemcell.com Follow us on Twitter: https://bit.ly/3jW20Gv Follow us on Instagram: https://bit.ly/3LrNkOs Like us on Facebook: https://bit.ly/32ern07 Follow us on LinkedIn: https://bit.ly/32uKovJ Subscribe to our YouTube channel: https://bit.ly/32f28ei