Скачать с ютуб Quantitative Estimation of Aminoacids by Ninhydrin Method - Amrita University в хорошем качестве

Quantitative Estimation of Aminoacids by Ninhydrin Method - Amrita University

▶ This video channel is developed by Amrita University's CREATE http://www.amrita.edu/create ▶ Subscribe @    / amritacreate      / amritavlab   ▶ Like us @   / createatamrita   ▶ For more Information @ http://vlab.amrita.edu/index.php?sub=... ▶ Amrita Virtual Lab Project website http://vlab.amrita.edu Amino acids are known to be the building block of all proteins. Twenty different amino acids are commonly found in proteins. Amino acids comprises of a carboxyl group and an amino group bonded to the same carbon atom (the α carbon).They differ from each other in their side chains (R groups),which vary in structure, size, electric charge and solubility in water. Hence their detection, quantification and identification in any sample constitute important steps in the study of proteins. Ninhydrin (triketohydrindene hydrate), a powerful oxidizing agent reacts with all amino acid between Ph 4 and 8 to give purple coloured compound. The reaction is also given by primary amino and ammonia, but without the liberation of CO2. Heating in the presence of Ninhydrin causes oxidative deamination of the alpha amino group amino acids and peptides with the concomitant reduction of Ninhydrin molecule. The reduced Ninhydrin react with ammonia and with a molecule of the oxidized Ninhydrin to yield coloured compound (Ruhemann's violet-blue complex).