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Affinity Chromatography Explained

This video introduces affinity chromatography, an essential laboratory method to isolate target biomolecules from complex mixtures. Whether you're a student, researcher, or simply curious about the science behind this technique, this video is for you! First, we show you how it works by taking advantage of the specific binding interactions between a target biomolecule and a complementary ligand immobilized on a stationary phase. Then, we explain the different components of the stationary phase. Finally, we walk you through the various steps of an affinity chromatography experiment. As an example, we use Immobilized Metal Affinity Chromatography (IMAC), one of the most common kinds of affinity chromatography and a method we apply in our laboratory daily. By the end of this video, you'll understand how to use affinity chromatography to isolate and purify target biomolecules from complex mixtures. However, if you would like to go into more depth on the topic, check out the video linked below: https://cube-biotech.com/knowledge/pr...