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Gel permeation chromatography | gel Filtration chromatography | Size exclusion chromatography

Gel permeation chromatography is also known as gel filtration or size exclusion chromatography. in gel permeation chromatography the separation of molecules occurs based on the size. The gel used have porous structure. small molecules easily gets trapped in the pores of the beads while the large molecules remains outside. hence when the mobile phase is passed, the large molecules moves fast down the column while the small molecules takes long time to move.