Скачать с ютуб Single-Unit Recording Explained! | Neuroscience Methods 101 в хорошем качестве

Single-Unit Recording Explained! | Neuroscience Methods 101

In this video we explain how single-unit recording (aka single-cell recording) works. With single-unit recording we can look at the activity of a single neuron in the brain. As such, it is distinctly different from other techniques such as EEG, MEG, and fMRI. However, for single-unit recording electrodes need to be inserted into the brain. Given the invasive nature, this research is mainly done in animals. But occassionally single-cell recording can be done in humans, if neurological patients receive such recording electrodes to monitor their disorder. Single-unit recording is an example of an extracellular recording technique. That means an electrode is placed next to a neuron (and not inserted into a neuron as is the case with intracellular recordings). The signal of an extracellular single-unit electrode show are flipped compared to the intracellular action potential. Since single-units often pick up signals from multiple cells, the neuronal firing needs to be organized. This step is called spike sorting. Since the shape of each neuron looks a little different from its neighbour, we can distinguish spikes from different cells by looking at the amplitude and width of the signal. Finally, the data can be represented in so-called raster plots. References/resources: Kamiński, J., Sullivan, S., Chung, J. M., Ross, I. B., Mamelak, A. N., & Rutishauser, U. (2017). Persistently active neurons in human medial frontal and medial temporal lobe support working memory. Nature neuroscience, 20(4), 590–601. https://doi.org/10.1038/nn.4509 Tseng, H. A., & Han, X. (2021). Distinct Spiking Patterns of Excitatory and Inhibitory Neurons and LFP Oscillations in Prefrontal Cortex During Sensory Discrimination. Frontiers in physiology, 12, 618307. https://doi.org/10.3389/fphys.2021.61... Cardin, J. A., Palmer, L. A., & Contreras, D. (2007). Stimulus feature selectivity in excitatory and inhibitory neurons in primary visual cortex. The Journal of neuroscience : the official journal of the Society for Neuroscience, 27(39), 10333–10344. https://doi.org/10.1523/JNEUROSCI.169... Graziane, N., Dong, Y. (2016). Extracellular and Intracellular Recordings. In: Electrophysiological Analysis of Synaptic Transmission. Neuromethods, vol 112. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-32... Hong, G., Lieber, C.M. Novel electrode technologies for neural recordings. Nat Rev Neurosci 20, 330–345 (2019). https://doi.org/10.1038/s41583-019-01... Johnson, L., Alekseichuk, I., Krieg, J., Doyle, A., Yu, Y., Vitek, J., Johnson, M., & Opitz, A. (2020). Dose-dependent effects of transcranial alternating current stimulation on spike timing in awake nonhuman primates. Science advances, 6(36), eaaz2747. https://doi.org/10.1126/sciadv.aaz2747 Narrated by: Miles Wischnewski USE OF STOCK IMAGES AND VIDEOS Occasionally we make use of stock images and videos (pixabay.com or pexels.com). We use these to make our videos more lively (better than looking at a blank screen). These stock videos should not be taken as an exact scientific reflection of the discussed content. In some cases they are not fully accurate. We try to have a high viewing pleasure, while at the same time create as little confusion as possible. Thus we aim to only use them when it is clear that they are not directly related to the discussed content. CHANNEL GOALS The Psyched! channel focuses on providing education about psychology and neuroscience. Here you learn all about human behavior, the mind and the brain. It is our goal to reach everyone, from people who are casually interested to academic researchers. As such we provide simple explanation videos as well as profound lectures. If you like our content, consider to subscribe! SOCIAL MEDIA LINKS Instagram:   / real.psyched   Twitter:   / mileswischnews1   Website: www.mileswischnewski.com