Скачать с ютуб How to Make Mushroom Culture Slants for Long Term Storage в хорошем качестве

How to Make Mushroom Culture Slants for Long Term Storage

In this video I show you how to make agar culture slants by allowing the agar to cool with the tube laying at an angle, resulting in a large surface area for spreading a culture. Agar slants are better suited to maintain stock cultures due to the capability of the slant containers to be capped or sealed, preventing the slant from drying out while also preventing contaminants from entering the media. Slant cultures are also manageable to store due to their small size. Some advantages over agar plates include less storage space used, less cell division, less drying out, and less chance of fruiting in storage. Agar Recipe: 10 grams of LME + 10 grams of Agar Agar powder + 500 ml boiling water. *Make sure to mix while hot and dissolve completely before pouring into the tubes. Make sure to work fast so that the agar does not solidify. Mushroom liquid culture and supplies: https://sporensprout.etsy.com Check out my social links! https://linktr.ee/sporensprout Relevant Links (Affiliate): Centrifuge tubes: https://amzn.to/3jYVcNV Light Malt Extract: https://amzn.to/3ihXV4C Agar Agar powder: https://amzn.to/3immIEU Micropore tape: https://amzn.to/3WaJhuK RTV Silicone: https://amzn.to/3jYVEvB Metal Hole Puncher: https://amzn.to/3Wk6c7a Parafilm: https://amzn.to/3WIKVEd Pressure Cooker: https://amzn.to/3XdhJVU Popsicle sticks: https://amzn.to/3ZdP8S3 Digital scale: https://amzn.to/3jLvBbe Disposable scalpels: https://amzn.to/3GnUZM0 Isopropyl alcohol: https://amzn.to/3GLV39u Butane torch: https://amzn.to/3Z8sQRw *clicking my affiliate links and making a purchase may result in me earning a small commission, thanks for supporting the channel 🍄 🍄 Join the new mushroom cultivation Discord community!   / discord   Chapters: 0:00 Culture Slant Intro 0:24 Supplies 0:53 Popsicle Sticks 1:00 Jar Lids 1:12 Making Agar 6:12 Sterilization 7:23 Making The Slant 10:11 Verify Clean Slants 10:52 Inoculation 14:55 Colonization 15:10 Outro Join the new exclusive member channel to get access to perks:    / @sporensprout   Music by: Chillpeach - Mushroom's Life:    • Видео