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How to Automatically Calculate RF Values for Western Blot/SDS-PAGE Gel Bands in Phoretix 1D 1 год назад


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How to Automatically Calculate RF Values for Western Blot/SDS-PAGE Gel Bands in Phoretix 1D

In this video, I'm going to tell you what the RF value is and how we can use Phoretix 1D to automatically calculate them. 00:00 - Intro 00:19 - What is RF? 01:10 - What is the relative front? 01:30 - Dye front and lane end explanation 02:00 - Detecting lanes 02:15 - Correcting lanes for frowning gels 03:20 - Defining the top of your lane 03:41 - Detecting bands 03:55 - Peaks of bands for measurement 04:20 - Turning on RF measurement 05:01 - How to interpret RF values 06:12 - How to get a free trial of Phoretix 1D For a free trial of Phoretix 1D to see how well it works with your images from your lab, please fill in the form here: https://totallab.com/products/phoreti... Phoretix 1D can provide quantitative analysis of blot and gel images, lateral flow test images, TLC (thin layer chromotography) images and more. See our other scientific software products here: https://totallab.com/products/ And if you want to get in touch with us here at TotalLab: Website: http://www.totallab.com/ LinkedIn:   / totallab-ltd   #westernblot #electrophoresis #SDSPAGE #tlc #quantification #immunoblot #software #imageanalysis #cliqs #totallab1d #imagej #21cfr #FDA #science #lifescience

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