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MALDI Biotyper® sirius and resistance detection

Presented By: Diana Kalinina, PhD Gerald Larrouy-Maumus, PhD Speaker Biography: Dr. Diana Kalinina joined Bruker in 2021 as Global Product Manager of MALDI Biotyper® systems. She started her career in academia developing various diagnostics solutions in plasma physics before joining the MALDI-TOF industry. She was involved in the development of several commercially available MALDI-TOF instruments before gradually moving into a Product Management role. Currently at Bruker she is responsible for the Bruker MALDI-TOF portfolio, from research-grade to routine solutions, within the business area Bruker Microbiology & Diagnostics, focusing to the MALDI Biotyper® solutions. Managing the complete product life cycle of the MALDI Biotyper® portfolio, including new product launches of instruments, accessories and consumables, is the major topic of her daily work. Dr. Gerald Larrouy-Maumus is a Senior Lecturer in Molecular Microbiology from the Department of Life Sciences, Faculty of Natural Sciences at the MRC-CMBI, Imperial College London. Over the last decade he has developed cutting edge expertise in biochemistry, lipidomics and metabolomics of bacterial pathogens. The major aim of Dr. Gerald Larrouy-Maumus’ laboratory is to understand how mycobacterial metabolic flexibility impacts drug resistance and immune persistence. In parallel, his lab is pioneering bacterial antibiotic susceptibility testing on intact bacteria using lipids for identification and read-out of Antimicrobial Resistance using MALDI-ToF, the workhorse of clinical microbiology labs worldwide. Since 2019, Dr. Gerald Larrouy-Maumus has established a strong collaboration with Bruker Daltonics in order to translate his research on colistin resistance towards the development of rapid diagnostics of drug resistant pathogens, using lipids - analyzed on the MALDI Biotyper sirius system - as a read-out. This has led to the new MBT Lipid Xtract™ Kit (RUO), launched in July 2021. Webinar: MALDI Biotyper® sirius and resistance detection Webinar Abstract: Dr. Diana Kalinina shall introduce the MALDI Biotyper® sirius platform and elaborate the already available as well as the newly introduced solutions, workflows and accessories that have been made available from Bruker Microbiology & Diagnostics. Additionally, the focus will be around the new MBT Lipid Xtract™ Kit (RUO) kit and related software, for colistin resistance detection. In addition to proteins, lipids are also abundant molecules in bacteria and could be used to unlock the challenges of microbial identification and antimicrobial susceptibility testing that analysis at proteins level cannot resolve. However, until now, their use in routine clinical diagnostics have not gained a deep interest due to their challenges associated with extractions and analysis. In this presentation, Dr. Gerald Larrouy-Maumus will present the latest advances in the detection of species-specific lipids by the MALDI Biotyper sirius system, their potential use in rapid diagnostics and illustrate the applicability of lipids in routine application by using the MBT Lipid Xtract™ Kit (RUO) and software for rapid detection of colistin-resistant E. coli. LabRoots on Social: Facebook:   / labrootsinc   Twitter:   / labroots   LinkedIn:   / labroots   Instagram:   / labrootsinc   Pinterest:   / labroots   SnapChat: labroots_inc