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Lecture 7b: Flow Cytometry

UCSD Extension School: Applied Immunology (BIOL-40371) Summer Quarter 2021 This lecture provides an introduction to flow cytometry techniques used for immunophenotyping both basic research and clinical patient samples. This lecture includes a discussion of sample flow plots used to diagnose various clinical pathologies, including B cell acute lymphoblastic leukemia (B cell ALL), severe combined immunodeficiency (SCID), perforin deficiency, and chronic granulomatous disease (CGD). All figures are either from Janeway's Immunobiology (9th ed.) where noted, or my own original figures.