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Finding the Right Primers: Using NCBI for RT-PCR Primer Design

Specializing in the design and troubleshooting of qPCR, mutagenesis, and cloning experiments at IDT, Scientific Applications Specialist, Adam Clore PhD, speaks about the intelligent selection of PCR primers. Topics include identifying transcript variants for your target of interest and selecting primers that amplify only specific transcripts. Adam also discusses the growing SNP database and the potential impact of SNPs on qPCR data, how to locate any SNPs that fall in your target amplicon, and the use of free NCBI and IDT tools to help you avoid them. About the Speaker: Adam Clore Adam Clore is a Scientific Applications Specialist at Integrated DNA Technologies. He received his BS in Microbiology and Chemistry from the University of Northern Iowa and his PhD in Biology from Portland State University where he studied the molecular genetics of Hyperthermophilic Archaea and their viruses. His postdoctoral research involved studying DNA damage from environmental toxins and repair pathways. His roles at IDT include assay design and troubleshooting, new product development, research, and scientific writing.