Скачать с ютуб How to perform an Oxidase test in microbiology for gonorrhea and non fermenters в хорошем качестве

How to perform an Oxidase test in microbiology for gonorrhea and non fermenters

An oxidase test is a microbiology procedure to detect cytochrome oxidase activity in bacteria. Cytochrome containing organisms produce an intracellular oxidase enzyme. This oxidase enzyme catalyzes the oxidation of cytochrome c. Organisms which contain cytochrome c as part of their respiratory chain are oxidase- positive and turn the reagent blue/purple. Organisms lacking cytochrome c as part of their respiratory chain do not oxidize the reagent, leaving it colorless within the limits of the test, and are oxidase-negative. The oxidase test can be used in the presumptive identification of Neisseria spp. and in the differentiation and identification of gram-negative bacilli. Oxidase-positive organisms should be examined by Gram stain to determine morphology and Gram reaction. Additional biochemical tests are recommended for complete identification. Use of a nichrome or other iron containing loop may yield false-positive reactions. Platinum loops are recommended. Most Haemophilus species are oxidase-positive. Less sensitive strips or reagents may yield false-negative results. Oxidase reactions of gram-negative bacilli should be determined on non-selective and non-differential media to ensure valid results. Also, colonies taken from media containing high levels of glucose may give false-negative reactions. It is recommended to use colonies that are 18-24 hours old. Older colonies will produce weaker reactions. Any color changes appearing after 20 seconds should be disregarded. To order OxiDrops™, oxidase test, 7ml Cat. No. Z119 view the link below: https://hardydiagnostics.com/z119 Contact us with any questions: https://hardydiagnostics.com/contact-us Hardy Diagnostics is your complete Microbiology supplier. https://hardydiagnostics.com/lab-supp... Check out our full line up of inoculating loops by clicking the link below. https://hardydiagnostics.com/lab-supp... See our complete list of catalogs in the link below. https://bit.ly/4cQgtjN Hardy Diagnostics is 100% employee owned and has been serving microbiologists for over 40 years. 00:00 Intro 00:08 History of Oxidase Test 01:13 What you will need 01:49 How to perform an Oxidase test 02:14 Observations and results 02:25 Alternative method 02:40 List of bacteria that will be positive for Oxidase 02:58 Closing info Follow us: LinkedIn -   / hardydiagnostics   Facebook -   / hardydiagnostics   Instagram -   / hardydiagnostics   X - https://X.com/HardyDiagnostic Filmed and Edited by Brian Tom #HardyDiagnostics #Oxidase #microbiology