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MaGIC and the Cellular Imaging and Histology Core at Rutgers NJMS present @scintica and its Real-time IntraVital Microscopy (IVM): In Vivo Cellular-level Imaging of Internal Organs in a Live Animal Seminar Abstract: Intravital microscopy is a unique imaging technique to visualize various in vivo cellular level dynamics such as cell trafficking, cell-to-cell or cell-to-microenvironment interactions in a live animal. Intravital imaging of cellular dynamics in a natural physiological microenvironment can provide unprecedented insights in the dynamic pathophysiology of human diseases those were impossible to obtain through conventional histological observation of ex vivo sample or in vitro culture system. During the last decade, the intravital microscopy has become a highly valuable, indispensable technique in wide areas of biomedical sciences such as immunology, neuroscience, developmental and tumor biology. Notably, in vivo visualizations of gene expression, protein activity, cell trafficking, cell-cell/cell-microenvironment interactions and various physiological responses to external stimuli have been successfully achieved. Additionally, it’s a unique tool for the development of new therapeutics and diagnostics by providing improved accuracy and reliability in in vivo target validation with delivery monitoring and efficacy assessment. It has been used to directly analyze the delivery and efficacy of new biopharmaceuticals such as antibodies, cell therapy, gene therapy, nucleic acids, and exosome in an in vivo microenvironment. In this talk, @ivimtechnology3209's All-in-One real-time intravital two-photon and confocal microscopy system will be introduced. The imaging system has been extensively optimized for in vivo cellular level imaging of internal organs in live animal model for various human diseases. It can acquire real-time multi-color sub-micron resolution microscopic images in a live animal model with automatic motion compensation, enabling direct imaging analysis of complex cancer immune-microenvironment consisted of various immune cells, stromal cells, vascular cells and extracellular matrix. Intravital imaging of various organs including skin, liver, spleen, pancreas, kidney, small intestine, colon, retina, lung, heart, lymph node, brain, and bone marrow will be briefly introduced. Subsequently, recent studies utilizing the real-time intravital imaging technique to investigate dynamic cellular-level pathophysiology of various human diseases and develop new therapeutics will be introduced. Chapters: 0:00 – Welcome and Introduction to the Cellular Imaging and Histology Core and Scintica Team 2:39 – What is Intravital Microscopy? 25:39 – IVIM Technology’s Product Lines 27:22 – Animal Motion Compensation 29:37 – Live Animal Maintenance 30:20 – Confocal vs. Two-Photon Microscopy 36:28 – IVM Studio, Intravital Image Processing 37:37 – Application Support 47:26 – Q&A To learn more about @scintica and @ivimtechnology3209: https://www.scintica.com/products/ivi... https://www.ivimtech.com/intravital-m... Scintica Videos: IVIM Microscopy: • IVIM Microscopy The Basics of Intravital Fluorescence Microscopy: • IVIM Webinar - The Basics of Intravi... Introducing the IVM Intravital Microscope: • IVIM Webinar 2 - Introducing the IVM ... Highlighting Imaging Examples Using the IVM Intravital Microscope: • IVIM Webinar 3- Highlighting Imaging ... Live Virtual Demonstration of the IVM System: • Webinar #4 Live Virtual Demonstration... -------------------------------------------------- For more information on upcoming MaGIC seminars: https://bioinformagic.io/events Follow MaGIC for more content: Twitter: / magic_analytics Instagram: / magic_analytics #scintica #ivimtechnology #intravitalmicroscopy