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Behind the Bench: Gel electrophoresis 2 года назад


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Behind the Bench: Gel electrophoresis

In Lab 4 of The Wolbachia Project, we will be visualizing the DNA from your arthropod sample that was amplified via PCR. This timelapse video will show you the steps to run gel electrophoresis, and The Wolbachia Project Database. The corresponding curriculum for this video can be found here: https://wolbachiaproject.org/electrop... To get involved with The Wolbachia Project, visit us at https://wolbachiaproject.org/ All of our curriculum is available under the Creative Commons Attribution-Non Commercial- No Derivatives International License. Contact info(at)wolbachiaproject(dot)org if you would like to make adaptations beyond the classroom. 0:00 Intro 0:13 Prepare materials & workspace 0:50 Prepare buffer 1:00 Cast gel 1:37 Prepare gel electrophoresis apparatus 3:14 Load the gel 3:48 Run the gel 4:37 Image gel 4:47 Clean up 5:16 Why upload pictures to the database? 5:30 Overview: adding data to the database 6:20 Outro Gel electrophoresis apparatus from MiniOne: https://theminione.com/electrophoresi... Music from Uppbeat: https://uppbeat.io/t/pecan-pie/superc... License code: JUQ43AEACJKBPK2P Music from Uppbeat: https://uppbeat.io/t/richard-smithson... License code: X4UFLMFHAXGLCPGM

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