Скачать с ютуб Measuring protein concentration and sample denaturation в хорошем качестве

Measuring protein concentration and sample denaturation

If you have questions or comments please go to the new channel Ciencia y Science and ask your questions there so that I can answer them.    / @cienciayscience   In this tutorial video I have included both 1) measuring protein concentration from sample lysates using the Pierce BCA protein assay and 2) Denaturing sample lysates using 4X Laemmli buffer Time stamp 0:00 Introduction 0:16 Pierce BCA protein assay from Thermo 0:43 Protocol/instructions from kit 1:42 Albumin standard I use to make my protein standards from Thermo 2:18 Loading protein standards and lysate samples into 96-well plate 11:11 Calculations for BCA reagents A and B 14:10 Loading 200uL of reagents A and B mixture 16:10 Incubate plate at 37C for 30 minutes 16:29 Read plate at 562nm using Cytation 5 19:46 Saving your data in excel 22:09 Calculations to determine sample volume for denaturation 27:01 Bio-Rad 4X Laemmli Sample Buffer instructions 29:40 Preparing sample lysates with 4X Laemmli buffer 33:48 Vortex samples, quick spin and heat denaturation at 90C.