Скачать с ютуб Presentation: Free floating IHC with multiple labeling and tyramide signal amplification в хорошем качестве

Presentation: Free floating IHC with multiple labeling and tyramide signal amplification

Link to companion demo video that shows how this process is done:    • Demo: Free-floating multi-label immun...   Link to website with relevant protocols and contact info: https://sites.google.com/view/kru-neu... This video is a presentation in which I explain brain mapping, pros and cons of acquiring microscope images of brain tissue at low magnification, depth of field, different approaches to immunohistochemistry (IHC), what tyramide signal amplification (TSA) is, why it is useful, and how to do successive rounds of it in different colors. Finally, despite being the first part of this video's name, the last segment of the video covers what free-floating tissue staining is and a bit on how to do it. Time stamps: 0:00 - Ideas on brain mapping, low magnification, and depth of field 14:47 - Different types of IHC methods 20:25 - Tyramide Signal Amplification (TSA), how it works, pros and cons 29:30 - How to do multiple rounds of tyramide conjugate labeling 37:03 - How free-floating staining works