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Struggling with subjective confluency estimations during your cell culture experiments? Learn from this microscopy tutorial how to perform consistent confluency measurements with Mateo TL, our new digital microscope. It increases your confidence in the success of downstream experiments. Get to know Mateo TL: https://fcld.ly/frqy8s8 ▬ Transcript ▬▬▬▬▬▬▬▬▬▬▬▬ Place your cells on the stage. In phase contrast (PH) mode, choose the desired magnification by rotating the objective nosepiece. Use the focus knob to obtain a sharp image of your sample. Find a region of interest. Start confluency check. Toggle between show and hide outline function to evaluate confluency calculation. If needed, adjust the sensitivity. Recalculate. Confirm confluency result by toggling between show and hide outline. Save result. The results are stored in the gallery. Go to the gallery, find a stored result, and double-click on it to see more details. Mateo TL Routine Cell Culture? Check! ▬ Follow us ▬▬▬▬▬▬▬▬▬▬▬▬ Discover us on LinkedIn: / leica-microsystems Find us on Facebook: / leicamicrosystems Follow us on Twitter: / leicamicro Follow us on Instagram: / leica.microsystems ▬ More Videos ▬▬▬▬▬▬▬▬▬▬▬▬ Subscribe @LeicaMicrosystemsTutorials All videos: • Mateo Tutorials ▬ About Leica Microsystems ▬▬▬▬▬▬▬▬▬▬▬▬ Leica Microsystems develops and manufactures microscopes and scientific instruments for the analysis of microstructures and nanostructures. Widely recognized for optical precision and innovative technology, the company is one of the market leaders in compound and stereo microscopy, digital microscopy, confocal laser scanning, and super-resolution microscopy with related imaging systems, electron microscopy sample preparation, and surgical microscopy.