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How to build the Spatial Light Modulator for the PUMA 3D printed microscope

Learn how to build the spatial light modulator (SLM) of the PUMA 3D printed microscope for advanced computer-controlled Fourier light filtration contrast methods. PUMA is an open source portable microscope with fluorescence, polarisation, dark ground, phase contrast, epi-illumination and other advanced features. CONTENTS ======== 00:08 Intro 01:29 Types of SLM 03:33 Pros and Cons of the PUMA SLM 06:53 WARNING! Dangers of making the SLM. 07:57 How to Build the SLM 29:50 How to insert and remove the SLM from a slot SUPPORT PUMA ============ PUMA is an open source microscopy project. You can help support the project by: 1. Become a Patron:   / pumamicroscope   2. Donate via PayPal: https://www.paypal.com/donate/?hosted... 3. Simply subscribing to this YouTube channel, like, comment and share these videos. 4. Spread the word - post the news about PUMA and link to this YouTube channel on your social media sites and any other outreach method (tell your friends, colleagues, students or teachers and lecturers about PUMA, for example). 5. Consider purchasing your optics and related supplies from our affiliated online optics store, OptArc.co.uk ( https://www.optarc.co.uk/ ) --- PARTS AND TOOLS =============== For the latest details see the 'Bill_of_Materials' (BoM) on the PUMA GitHub page ( https://github.com/TadPath/PUMA ). The following notes explain the changes made while preparing this video. These changes have been on GitHub prior to release of this video. 3D Printed Models Updated for this Video ------------------------------------------------------- The below changes were made and uploaded to GitHub on 17th May 2022) Dominus: - Condenser_23_30 - Improved 30 mm lens fitment and screw head cut-outs. - Condenser_base - Modified to have a circular diaphragm over the thread. This provides a guide for any filter slider (including the SLM) and prevents the front of the filter getting stuck at the far end. - Cnd_to_UC - Modified to have the top thread shortened by 0.5 mm. This is to ensure it does not contact the above-mentioned diaphragm on the condenser base and raise the diaphragm thereby potentially impeding the passage of a filter / SLM - Cnd_mirror_holder_socket - Modified to have the top thread shortened by 0.5 mm (same reason as for the Cnd_to_UC above). Non-Printed Parts and Tools --------------------------- No changes were introduced with this video. For non-printed parts see the BoM. Tools include: - Safety goggles and gloves - flat bladed knife e.g. Stanley knife - surgical scalpel (No.4 handle and No 22 blade) or similar craft knife - Scissors - Some soft packing foam or polystyrene to rest TFT screen on during cutting - Fine toothed craft saw to cut the PCB (I use a Zona SA35/050 52-Teeth Razor Saw) - two part epoxy resin - 1.5 mm Allen key - solder and soldering iron, stand, sponge, etc. - long nosed pliars - TFT screen driver and power supply of your choice. You must ensure you use the right voltage equipment for your model of TFT. FOR FURTHER INFORMATION ======================= 1. Open access write-up about PUMA on the FreeCAD blog: https://blog.freecad.org/2023/02/13/a... 2. The official PUMA GitHub page where you can download the specs and source files to build or customise your own PUMA system: https://github.com/TadPath/PUMA 3. The scientific peer reviewed publication on PUMA that was published in 2021 in the Journal of Microscopy (a journal of the Royal Microscopical Society) available here: https://pubmed.ncbi.nlm.nih.gov/34151... https://onlinelibrary.wiley.com/doi/1... PJT 04/06/22