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Dam and Dcm methylation (restriction independent methyl-transferases) affects restriction enzymes

References/Resources:   / the_crux   This video discusses restriction independent methyl-transferases like dam and dcm methylase - their biological function and how they affect restriction enzyme behavior. Apart from Dam and Dcm methyl-transferases, we discuss EcoK1, mcrABC and mrr endonuclease as well. All this is done to recognize the importance of understanding DNA methylases and their impact on molecular cloning - four examples are discussed. Jump to your favorite section: 00:00 Outline 00:41 Dam methylase 01:42 Dcm methylase 02:57 EcoKI methylase 04:21 Dam Dcm affect restriction enzymes 06:09 Dam Dcm affect transformation 07:02 Dam Dcm affect repetitive DNA 07:32 mcrABC and mrr endonuclease 09:30 Dam Dcm are not important Patreon Page:   / the_crux   Suggested videos: Restriction Enzymes:    • Restriction Enzymes in Molecular Cloning   Must watch playlists All videos on Genetic Engineering:    • Genetic Engineering (Genes to Genomes)   All videos on DNA Replication:    • All about DNA Replication!   All videos on Transcription:    • All about Transcription!   All videos on Translation:    • All about Translation!   Do leave a LIKE if you find this content useful! COMMENT your questions, suggestions, and thoughts :) And I hope you have SUBSCRIBED because there is a lot more coming! :D I appreciate your support.