Скачать с ютуб Cloning with Restriction Enzymes (5 Levels) - Traditional Molecular Cloning в хорошем качестве

Cloning with Restriction Enzymes (5 Levels) - Traditional Molecular Cloning

References/Resources:   / the_crux   Playlist - Genetic Engineering:    • Genetic Engineering (Genes to Genomes)   Molecular Cloning with restriction enzymes can be very easy or very challenging. This video discusses the fundamental considerations of traditional restriction enzyme based molecular cloning. We discuss sticky and blunt ends, the process of blunting, and how all of this (among other things) can be used to understand the different levels of molecular cloning. Doesn’t matter if you are doing subcloning or a PCR based cloning, you will eventually find yourself in one of the levels. Jump to your favorite section: 00:00 Cloning and Tetris 00:56 Sticky ends 01:18 Where do you cut? 02:29 Blunt ends 02:49 Cheating with Klenow 04:42 Level 1 07:04 Level 2 08:21 Level 3 11:57 Level 4 14:25 Level 5 Patreon Page:   / the_crux   Suggested videos: Plasmids (pUC19, pBR322, etc.):    • Plasmid and Phagemid cloning vector -...   Yeast Cloning Vectors:    • Lambda Phage and Cosmid cloning vecto...   BACs/Fosmids:    • F plasmid, BACs, Fosmid cloning vecto...   Lambda/Cosmids:    • Lambda Phage and Cosmid cloning vecto...   Must watch playlists All videos on Genetic Engineering:    • Genetic Engineering (Genes to Genomes)   All videos on DNA Replication:    • All about DNA Replication!   All videos on Transcription:    • All about Transcription!   All videos on Translation:    • All about Translation!   Consider supporting the growth of this channel by becoming a Patron:   / the_crux   I appreciate your support :)