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Seminar Abstract: @levitasbio1505 has developed Levitation Technology to address the challenges of dead cells and debris contaminants that often lead to deleterious effects on downstream assays. Unlike other cell separation methods, our LeviCell platform does not require labeling or staining and does not cause cellular stress or processing-induced activation. Healthy cells are enriched based on their intrinsic properties coupled with magnetic forces existing in the sample solution. This results in a single-cell suspension ready for immediate sequencing library preparation or subsequent processing, producing molecular profiles free of sample prep-induced gene expression artifacts. In addition, we have developed reagents that extend the technology to yield viable cell samples enriched with the desired cell types of interest. Our unique levitation process rescues and unlocks otherwise difficult-to-work-with, limited (less than 100k cells), and low viability (less than 40%) samples by removing contaminants and maximizing cell recovery. Chapters: 0:00 – Welcome and Introduction to the Levitas Team 3:02 – Challenges to scRNA preps 5:27 – What Levitas enables 8:26 – Processing precious samples 10:12 – Levitas Workflow 17:16 – Improving challenging samples 31:13 – Case study: Low cell yields from fragile gut samples 37:50 – Case study: Organoids 39:06 – Case study: CRISPR treatment enrichment 46:24 – Q&A To learn more about Levitas: Levitas – https://levitasbio.com/ EOS Platform – https://levitasbio.com/eos/ -------------------------------------------------- For more information on upcoming MaGIC seminars: https://bioinformagic.io/events Follow MaGIC for more content: Twitter: / magic_analytics Instagram: / magic_analytics #celllevitation #levitas #singlecell #scRNA